# glass frog ![[250px-cochranella_susatamai03.jpg|300]] rulyrana susatamai_ scientific classification kingdom: animalia phylum: chordata class: amphibia order: anura suborder: neobatrachia superfamily: hyloidea family: centrolenidae taylor 1951 subfamilies **+** hyalinobatrachinae- centroleninae ![[centrolenidae_range.png]] distribution of centrolenidae (in black]] the glass frogs belong to the amphibian family centrolenidae (order anura) native to the central american and colombian rainforests. the general background coloration of most glass frogs is primarily lime green the abdominal skin of some members of this family is transparent and translucent giving the glass frog its common name. the internal viscera including the heart liver and gastrointestinal tract are visible through the skin. when active ir blood makes them visible; when sleeping most of the blood is concealed in the liver hiding them. glass frogs are arboreal living mainly in trees feeding on small insects and only coming out for mating season. ir transparency conceals them very effectively when sleeping on a green leaf as they habitually do. however climate change and habitat fragmentation have been threatening the survival rates of the family. when resting glass frogs concentrate most of ir red blood cells in ir liver increasing transparency and improving ir camouflage the first described species of centrolenidae was the "giant" centrolene geckoideum named by marcos jimenez de la espada in 1872 based on a specimen collected in northeastern ecuador. several species were described in subsequent years by different herpetologists (including g. a. boulenger g. k. noble and e. h. taylor) but usually placed together with the tree frogs in the genera hylella or hyla the family centrolenidae was proposed by edward h. taylor in 1945. between the 1950s and 1970s most species of glass frogs were known from central america particularly from costa rica and panama where taylor julia f. and jay m. savage extensively worked and just a few species were known to occur in south america. in 1973 john d. lynch and william e. duellman published a large revision of the glass frogs from ecuador showing the species richness of centrolenidae was particularly concentrated in the andes. later contributions by authors such as juan rivero savage william duellman john d. lynch pedro ruiz-carranza and jose ayarzagüena increased the number of described taxa especially from central america venezuela colombia ecuador and peru the evolutionary relationships biogeography and character evolution of centrolenidae were discussed by guayasamin et al. (2008) glass frogs originated in south america and dispersed multiple times into central america. character evolution seems to be complex with multiple gains and/or losses of humeral spines reduced hand webbing and complete ventral transparency. researched by santiago (2009) evolution and speciation on glass frogs has shown that ecological gradient and isolation have a role in speciation and divergence in glass frogs by comparing glass frogs mitochondrial dna. glass frogs have expanded from the guiana shield to other rainforest and diversified further. they evolved to be able to survive and fit in with ir environment the taxonomical classification of the glass frogs has been problematic. in 1991 after a major revision of the species and taxonomic characters the herpetologists pedro ruiz-carranza and john d. lynch published a proposal for a taxonomic classification of the centrolenidae based on cladistic principles and defining monophyletic groups. that paper was the first of a series of contributions dealing with the glass frogs from colombia that led them to describe almost 50 species of glass frogs. the genus centrolene was proposed to include the species with a humeral spine in adult males and the genus hyalinobatrachium to include the species with a bulbous liver. however they left a heterogeneous group of species in the genus cochranella defined just by lacking a humeral spine and a bulbous liver. since the publication of the extensive revision of the colombian glass frogs several other publications have dealt with the glass frogs from venezuela costa rica and ecuador in 2006 the genus nymphargus was erected for the species with basal webbing among outer fingers (part of the previous cochranella ocellata species group) four genera (centrolene cochranella hyalinobatrachium nymphargus) have been shown to be poly- or paraphyletic and recently a new taxonomy has been proposed (see below) the family centrolenidae is a clade of anurans. previously the family was considered closely related to the family hylidae; however recent phylogenetic studies have placed them (and ir sister taxon the family allophrynidae) closer to the family leptodactylidae the monophyly of centrolenidae is supported by morphological and behavioral characters including: 1) presence of a dilated process on the medial side of the third metacarpal (an apparently unique synapomorphy); 2) ventral origin of the musculus flexor teres digiti iii relative to the musculus transversi metacarpi i; 3) terminal phalanges t-shaped; 4) exotroph lotic burrower/fossorial tadpoles with a vermiform body and dorsal c-shaped eyes that live buried within leaf packs in still or flowing water systems; and 5) eggs clutches deposited outside of water on vegetation or rocks above still or flowing water systems. several molecular synapomorphies also support the monophyly of the clade the taxonomic classification of the centrolenidae was recently modified. the family now contains two subfamilies and 12 genera **+** subfamily centroleninae - genus centrolene jimenez de la espada 1872 - genus chimerella guayasamin castroviejo trueb ayarzagüena rada vilá 2009 - genus cochranella taylor 1951 - genus espadarana guayasamin castroviejo trueb ayarzagüena rada vilá 2009 - genus nymphargus cisneros-heredia & mcdiarmid 2007 - genus rulyrana guayasamin castroviejo trueb ayarzagüena rada vilá 2009 - genus sachatamia guayasamin castroviejo trueb ayarzagüena rada vilá 2009 - genus teratohyla taylor 1951 - genus vitreorana guayasamin castroviejo trueb ayarzagüena rada vilá 2009 - genus incertae sedis - "_centrolene_" acanthidiocephalum (ruiz-carranza and lynch 1989) - "_centrolene_" azulae (flores and mcdiarmid 1989) - "_centrolene_" guanacarum ruiz-carranza and lynch 1995 - "_centrolene_" medemi (cochran and goin 1970) - "_centrolene_" petrophilum ruiz-carranza and lynch 1991 - "_centrolene_" quindianum ruiz-carranza and lynch 1995 - "_centrolene_" robledoi ruiz-carranza and lynch 1995 - "_cochranella_" duidaeana (ayarzagüena 1992) - "_cochranella_" euhystrix (cadle and mcdiarmid 1990) - "_cochranella_" geijskesi (goin 1966) - "_cochranella_" megista (rivero 1985) - "_cochranella_" ramirezi ruiz-carranza and lynch 1991 - "_cochranella_" riveroi (ayarzagüena 1992) - "_cochranella_" xanthocheridia ruiz-carranza and lynch 1995 **+** subfamily hyalinobatrachinae - genus celsiella guayasamin castroviejo trueb ayarzagüena rada vilá 2009 - genus hyalinobatrachium ruiz-carranza & lynch 1991 - "true" glass frogs **+** subfamily incertae sedis - ikakogi guayasamin castroviejo trueb ayarzagüena rada vilá 2009 ![[250px-glass_frog2.jpg|300]] ![[glass_frog_cr.jpg|300]] the evolutionary advantage of a partly clear skin and an opaque back was a mystery as it did not seem to be effective as camouflage. it was found that the colour of the frog's body changed little against darker or lighter foliage but the legs were more translucent and consequently changed in brightness. by resting with the translucent legs surrounding the body the frog's edge appears softer with less brightness gradient from the leaf to the legs and from the legs to the body making the outline less noticeable. this camouflage phenomenon in which the frog's edges are softened to match the relative brightness of its surroundings is referred to as edge diffusion. herpetology researchers study the pros and cons of transparency in glass frogs it was established that the transparency offers more than regular color changes in the skin itself through limited pigments. experiments with computer-generated images and gelatine models of opaque and translucent frogs found that the translucent frogs were less visible and were attacked by birds significantly less often. photographs of the frogs were taken both at nighttime and during the day results showed little to no visibility of the frogs on any leaves in the daytime or nighttime. it was found in 2022 that these frogs have the ability to conceal red blood cells concentrated inside ir livers increasing transparency when they are vulnerable. while this would cause massive clotting in most animals (including humans) glass frogs are able to regulate the location density and packing of red cells without clotting. the findings could advance medical understanding of dangerous blood clotting ![[glass_frogs.jpg|300]] a group of glass frogs glass frogs are generally small ranging from 3 - 7.5 cm (1.2 - 3.0 in) in length. they appear light green in color over most of ir bodies except for the skin along the lower surface of the body and legs which are transparent or translucent. the glass frog's transparent skin allows an external view of the viscera—the internal organs present in the body's main cavity - making it so observers can witness the frog's internal processes such as the heart beating and pumping blood through its arteries. patterning of glass frogs is varied amongst different species while some appear as a uniform green color others display spots that range from yellow to white mimicking the coloration of ir eggs ir digit tips are expanded allowing them to climb thus allowing most to live in elevated areas along forest streams such as trees and shrubs glass frogs are similar in appearance to some green frogs of the genus eleutherodactylus and to some tree frogs of the family hylidae. however hylid tree frogs have eyes that face to the side whilst those of glass frogs face forward two members of the glass-frog family centrolenidae: centrolenella fleischmanni now called hyalinobatrachium fleischmanni and c. prosoblepon and of the hylid subfamily phyllomedusinae: agalychnis moreletii and pachymedusa dacnicolor reflect near-infrared light (700 to 900 nanometers) when examined by infrared color photography. infrared reflectance may confer adaptive advantage to these arboreal frogs both in thermoregulation and infrared cryptic coloration. an endangered species of glass frog found in peru was compared to the n. mixomaculatus species and the following results were recorded: no humeral spine no webbed fingers between ii and iii finger i shorter than ii no vomerine teeth no ulnar and tarsal tubercles or folds no white pigment in the visceral or hepatic peritonea and differing coloration and spots the glass frog life cycle begins with a male and female mating on a leaf overhanging a stream after which the female lays eggs and the male guards them. once the leaf falls into the stream the eggs hatch from 10 to 20 days often during heavy rainfall. they then undergo metamorphosis in the water and on the stream's edge eventually transforming into adult frogs that return to the terrestrial environment near streams mating begins by the call of a male tree frog who is perched either on the underside or top of a leaf over a lake edge or a stream. once a female has responded to the male's call mating begins on the leaf in the amplexus physical position in which the male wraps ir arms around the female and attaches himself to ir back. once the physical mating process has concluded the female produces ir eggs onto the leaf before departing leaving the male to defend the newly-laid eggs against predators centrolenidae is a species that has long-term parental care males guard the clutch for various days after the eggs are laid. environmental aspects also play into the amount of time the male glass frog tends to the young such as rainfall or wind. female post-oviposition care is most often based on body conditions whether or not they is able to fend for herself will tell how long after ir eggs are laid that they will remain by the clutch. males will occasionally call for and mate with other females on the same leaf establishing a multitude of different developmentally-staged egg clutches to guard once the tadpoles the frog aquatic larval stage have been hatched they fall from ir original position on the leaf into the water below. when living in the water the tadpoles feed on the leaf litter and streamside detritus until undergoing metamorphosis to become a froglet a main predator on the glass frog in its tadpole stage are "frog flies" which lay ir eggs within the frog eggs; after hatching the maggots feed on the embryos of the glass frogs glass frog behaviors to avoid predation vary from species to species as well as circumstances. hyalinobatrachium iaspidiense was observed having a flattened body posture to avoid predation after disturbing the frog it propped up into a sitting position. another male h. iaspidiense was observed protecting an egg clutch with a body positioning of extending all limbs and lifting its body from the leaf in addition to predation glass frog populations are experiencing declines due to habitat loss. deforestation has been increasing in south america the natural habitat of the glass frog. this has led to less tree cover and nowhere for these frogs to go. furthermore deforestation may also result in alterations to local climate patterns. as a result researchers have proposed that these frogs be classified as critically endangered rather than merely endangered all glass frogs are protected under the convention on international trade in endangered species (cites) meaning that international trade (including in parts and derivatives) is regulated by the cites permitting system the centrolenidae are a diverse family distributed from southern mexico to panama and through the andes from venezuela and the island of tobago to bolivia with some species in the amazon and orinoco river basins the guiana shield region southeastern brazil and northern argentina the biggest threats they have are deforestation invasive species pollution habitat loss and illegal pet trade. these many threats have led to a decline in the population of this species glass frogs are mostly arboreal. they live along rivers and streams during the breeding season and are particularly diverse in montane cloud forests of central and south america although some species occur also in amazon and chocóan rainforest and semideciduous forests hyalinobatrachium valerioi glass frogs are carnivores ir diet mainly including small insects like crickets moths flies spiders and other smaller frogs the eggs are usually deposited on the leaves of trees or shrubs hanging over the running water of mountain streams creeks and small rivers. one species leaves its eggs over stones close to waterfalls. the method of egg-laying on the leaf varies between species. the males usually call from leaves close to ir egg clutches. these eggs are less vulnerable to predators than those laid within water but are affected by the parasitic maggots of some fly species. some glass frogs show parental care: in many species glass frog females brood ir eggs during the night the eggs are fertilized which improves the survival of the eggs while in almost a third of species glass frog males stay on guard for much longer periods. after they hatch the tadpoles fall into the waters below. the tadpoles are elongated with powerful tails and low fins suited for fast-flowing water. outside of the breeding season some species live in the canopy the majority of amphibians use cutaneous respiration or the process of breathing through the skin. due to the importance of the skin amphibians are very sensitive to what goes through ir permeable skin the stratum corneum is the main skin barrier which is much thinner than other classes such as mammals or birds. chemicals and high amounts of elements in water or rainfall may disturb frogs' health and possibly lives look up glass frog in wiktionary the free dictionary **+** amphibian species of the world **+** centrolenidae in amphibiaweb ecuador **+** centrolenidae in amphibiaweb **+** glassfrogs (centrolenidae) project **+** centrolenidae in the tree of life site **+** centrolenidae in itis **+** research on centrolenidae **+** centrolenidae in livingunderworld.org **+** centrolenidae in animal diversity web **+** centrolenidae en infonatura archived 2012-04-09 at the wayback machine **+** fleischmann's glass frog at national geographic archived 2007-10-28 at the wayback machine **+** various frog species // republic of bob